Additional file 6: of Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength

Inefficiently spliced introns and exons that are differentially used do not have weaker 3′ splice sites. a Inefficiently spliced introns do not have weaker 3′ splice sites. A set of 200 retained introns (RI) were selected based on fold change differences (see “Materials and methods”), and non-retained introns (NRI) within the same set of genes were used as a contrast. A 3′ splice site strength analysis was then carried out as described in “Materials and methods” for each subset. b Motif enrichment analysis on the same set of genes shows that the most frequently identified motifs correspond to the consensus 3′ splice site sequences for both retained (RI) and non-retained introns (NRI) and there is no significant change in the percentage of targets with such motifs. c Differentially used exons do not have weaker 3′ splice sites. A 3′ splice site strength analysis was carried out as described in “Materials and methods” for each subset. (PDF 63 kb)