Additional file 6: of Performance evaluation of commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier

Analysis of UMI errors. (a) The frequency of reads tagged with erroneous UMIs was estimated by UMI-tools. (b) Sequencing metrics obtained without UMIs, with UMIs, and with error-corrected UMIs using UMI-tools. The stacked bar plot shows the fractions of filtered reads (i.e., unaligned, duplicated, and off-target reads) and reads left after filtering (i.e., on-target) during raw data processing for five commercial kits with and without UMIs for deduplication. (JPG 149 kb)