Additional file 6: of Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Table S1, Figure S3. Effect on expression and enzyme activity of mtg gene copies. Table S1. The MTG activity in different copy strains. Figure S6. Detection of protein expression in strains with different mtg copy by Western blotting. The protein (MTG) in 20 μl of culture supernatant were separated by Western blot analysis (anti-MTG). Lane 1–3: mtg-2c, lane 4–6: mtg-3c, lane 7–9: mtg-6c. (ZIP 48 kb)