Additional file 6: of Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis

Figure S3. T-DNA organizations in contructs used in this study. All binary vectors have the same pPZP200 backbone [84]. (A) pEC3Pxxx-HPT3. (B) pEC3GPD-GUS. LB: left border of T-DNA; RB: right border of T-DNA; Pxxx represents three glyceraldehydes-3-phosphate dehydrogenase promoters from A. nidulans (P gpdA ), U. maydis (P gpd ) and R. toruloides (P GPD1 ) and the tranlation elongation factor promoter from A. gossypii (P tef ). hpt-3: codon-optimized hygromycin resistance gene based on the codon usage bias in R. toruloides; GUS: E. coli β-glucuronidase gene; T 35S : terminator of cauliflower mosaic virus 35S gene; Tnos: terminator of A. tumefaciens nopaline synthase gene; T tef : terminator of A. gossypii translation elongation factor gene; T cyc1 : Terminator of S. cerevisiae iso-1-cytochrome C gene. The labeled restriction enzymes are unique cutting sites in the plasmid. (PDF 56 kb)