Additional file 6: of Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Figure S5. Further detection of the potential off-target effects of sgRNA17–6. NC indicates the negative controls. Untransfected cells were used as negative controls. OTS indicates the experimental groups transfected with each pX458-sgRNAs. M, DNA marker. Red arrowheads indicate the expected cleaved bands by T7E1. (JPG 1060 kb)