Additional file 6: of Growth environment and organ specific variation in in-vitro cytoprotective activities of Picea mariana in PC12 cells exposed to glucose toxicity: a plant used for treatment of diabetes symptoms by the Cree of Eeyou Istchee (Quebec, Canada)

Figure S6. Taxifolin protects PC12 cells from high glucose-mediated death. Exposure to glucose toxicity produced a significant loss in cell viability compared to normal glucose conditions as assessed by mitochondrial dehydrogenase activity measured by cleavage of the formazan dye WST [left panel, ** denotes a significant difference (p < 0.01) relative to normal glucose control, Student’s t-test, n = 16]. Three concentrations of taxifolin (0.8, 1.6 and 3.2 μM) were administered to high glucose-treated cells. Cytoprotective activity was assessed as described in methods section. *denote significant differences (p < 0.05) between high glucose and taxifolin sample (ANOVA, post-hoc Dunnett’s t-test, n = 10–15). Data are reported as the mean ± SEM. (PDF 618 kb)