Additional file 6: of Fecal Aliquot Straw Technique (FAST) allows for easy and reproducible subsampling: assessing interpersonal variation in trimethylamine-N-oxide (TMAO) accumulation

Figure S4. TMAO accumulation is consistent for mice colonized with different straw aliquots of the same sample. Two human communities stored by FAST (sub.1 and sub.8) were transplanted by gavage (G) into germ-free B6 female mice 2–3 times (G1, G2, or G3) months apart. Following 2 weeks on a standard chow diet, mice were transitioned to a defined diet containing 1% choline for 2 weeks. Serum was collected from non-fasted animals and TMAO quantified by HPLC-MS/MS. Bars represent mean ± standard error (n = 2–4 mice per community). (PDF 30 kb)