Additional file 6: of Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer

Figure S3. related to Fig. 5. Intercellular transfer of miR-128-3p by 128-Exo sensitized CRC cells to oxaliplatin agents. A. Internalization of exosomes derived from FHC-128 cells. Labelled 128-exo (green fluorescent dye, PKH67) were uptake by HCT116OxR (DAPI-labelled) cells. B. RT-qPCR analysis of miR-128-3p in HT29OxR cells pre-incubated with indicated factors. C. CCK8 assay of HT29OxR cells pre-incubated with indicated factors for 48 h followed by oxaliplatin treatment at indicated concentrations. D. Flow cytometry apoptosis assay of HT29OxR cells pre-incubated with indicated factors for 48 h followed by oxaliplatin treatment (30 μM) for 24 h. E. A representative scatter-gram of Annexin V/PI potential test for HCT116OxR (upper) and HT29OxR (lower) cell apoptosis. F. Exosomes were imaged using electron microscopy. Scale bar = 200 nm. G. RT-qPCR assay was performed to detect miR-128-3p expression in HCT116OxR cells following various treatments. H. CCK8 assay of HCT116OxR cells pre-incubated with indicated factors for 48 h followed by oxaliplatin treatment at indicated concentrations. (TIF 1395 kb)