Additional file 6: of Evolution of strigolactone receptors by gradual neo-functionalization of KAI2 paralogues

Testing the robustness of the D14/KAI2 reconstruction. Cladograms depicting the outcome of maximum likelihood analysis (implemented in PhyML) on the D14/KAI2 dataset (minus charophyte and lycophyte KAI2 sequences) when each DDK clade is removed in turn (as indicated below the tree). All trees were rooted with liverwort KAI2A sequences. The 10 trees are fundamentally similar, placing all eu-KAI2 sequences (coloured dark blue) + liverwort KAI2B (indicated by *) as a grade relative to the remainder of the DDK clade (coloured as in Fig. 1). The branching order within the remainder of the DDK clade is relatively stable, although some clades swap positions, here indicated by a double-headed arrow. Most often gymnosperm DLK4 becomes sister to gymnosperm D14 rather than angiosperm D14, or the lycophyte and monilophyte DDK sequences swap positions. (PNG 996 kb)