Additional file 6 of Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells

Additional File 6. Conservation of sequence, chromatin state and function of ECR65 and ECR9. (A) The entirety of chick ECR65 (purple bar) aligns to open chromatin in the chick genome. The homologous mouse sequence (grey bar with red lines) only partly aligns to the open chromatin region in the mouse. Mouse ECR65 (long black bar) is a longer region of open chromatin. Regions 2 and 6 (small labelled black bars) are conserved between both Mouse ECR65 and Chick ECR65. Insets show zoomed out genomic area to include surrounding closed chromatin. (B) Mouse ECR65::GFP was electroporated into E5 chick retina along with ThrbCRM1::AU1 and cultured for 18–22 h before harvest and immunohistochemistry. Retinae were stained for GFP, AU1 and DAPI to examine overlap between GFP and AU1. Scale bar shown in last panel represents 50 μm and applies to all. (C) Chromatin accessibility at the ECR9 region in the mouse E12.5 retina. The thick black bar depicts the mouse ECR9 region, the grey bars represent the regions of homology to the chicken, and the thin black bars represent motifs identified in the mouse ECR9 sequence.