13058_2016_781_MOESM6_ESM.jpg (1.01 MB)
Additional file 6: Figure S6. of MCL-1 inhibition provides a new way to suppress breast cancer metastasis and increase sensitivity to dasatinib
figure
posted on 2016-12-08, 05:00 authored by Adelaide I. J. Young, Andrew Law, Lesley Castillo, Sabrina Chong, Hayley Cullen, Martin Koehler, Sebastian Herzog, Tilman Brummer, Erinna Lee, Walter Fairlie, Morghan Lucas, David Herrmann, Amr Allam, Paul Timpson, D. Watkins, Ewan Millar, Sandra O’Toole, David Gallego-Ortega, Christopher Ormandy, Samantha Oakesshowing that MCL-1 antagonism and Dasatinib treatment induced apoptosis in MDA-MB-468-2A cells but not MDA-MB-231-2A cells when grown in 2D monolayer cultures. Bar graphs depicting the average fraction of apoptotic cells (total Annexin V-positive by flow cytometry) as indicated after 24 hours after treatment with vehicle, DOX, 5 μM A1210477 and 5 μM UMI-77 alone and in combination with 1 μM dasatinib after 24 hours. All graphs and western blots are the average of three independent experiments. Bars indicate statistically significant groups, p value paired t tests. (JPG 1029 kb)
