Additional file 6: Figure S6. of Enhanced immunity in a mouse model of malignant glioma is mediated by a therapeutic ketogenic diet

Gating strategies for cytokine expression of T regulatory cells and NK cells. Tumor-infiltrating lymphocytes isolated from gliomas from mice fed KD versus SD were cultured alone or in the presence of GL261-Luc2 tumor cells. Analysis of IL-10 + Treg cells (A), IFNγ + NK cells (B), and TNF + NK cells (C) was performed. Percentage of positive cells indicated on plots. (TIF 10675 kb)