Additional file 6: Figure S5. of Rp58 and p27kip1 coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex

Forced expression of p27kip1 enhances radial migration and alters the morphological characteristics of embryonic cortical neurons. (A-B) Forced expression of p27kip1 leads to enhanced radial migration, observed as a significant increase in the proportion of cells arriving in the CP (F2,12 = 7.245, p = 0.0085; Two-way ANOVA followed by Bonferroni’s posthoc t-test; *p < 0.05) (C). (D) The intracortical positioning of p27kip1-overexpressing cells is also affected, observed as a significant increase in the proportion of cells within the upper CP (uCP) (F2,12 = 2.645, p = 0.118; Two-way ANOVA followed by Bonferroni’s posthoc t-test; *p < 0.05). (E-F) Representative images of IZ neurons within each treatment condition. (G) The distribution of cell shapes within the IZ are not significantly affected by p27kip1 overexpression (F2,24 = 1.8, p = 0.194; Two-way ANOVA. (H-J) Representative images of CP neurons within each treatment condition. Pie charts representing the lengths of the leading processes of uni/bipolar shaped neurons within the CP are represented for each treatment condition. (J) There is a significant interaction between the distribution of CP cell shapes and p27kip1 overexpression (F2,27 = 5.0, p = 0.0147; Two-way ANOVA), but the differences for a given cell shape was not significantly different upon posthoc t-testing. (K) The leading processes of p27kip1-overexpressing neurons were significantly shorter when compared with control (p < 0.0001 student’s t-test, two-tailed; n > 285 neurons per condition). Scale bars represent 100 μm (B) and 20 μm (F, I) respectively. (PDF 331 kb)