Additional file 6: Figure S5. of Identification and characterization of a minisatellite contained within a novel miniature inverted-repeat transposable element (MITE) of Porphyromonas gingivalis
2015-10-06T05:00:00Z (GMT) by
X-gal and ONPG assays of promoter capabilities of BrickBuilt_5 based on lacZ promoter probe constructs. Promoter-less lacZ vectors pCB182 and pCB192 give no apparent β –Galactosidase activity. BrickBuilt_5 leader and tail oligos (Eurofins Operon) were cloned into vector pCB192. Full-length BrickBuilt_5 was cloned into pCB192, ‘Normal’, with the tail segment of the element upstream of lacZ facing in the same orientation (tail abutting lacZ). Full-length BrickBuilt_5 was cloned into pCB182, ‘Reverse’, with the leader segment of the element upstream of lacZ facing in the same orientation (flipped leader abutting lacZ). X-gal activity visualized after 24 h incubation. Top of two liquid assays of ONPG activity visualized after 3 h incubation; bottom after 24 h incubation.