Additional file 6: Figure S5. of Evolutionary and functional analysis of mulberry type III polyketide synthases

In vivo characterization of MnPKS1 and MnPKS2 by coexpression with 4-coumaroyl-CoA ligase in Escherichia coli. Mn4CL: E. coli-expressed Mn4CL. Mn4CL+MnPKS1: E. coli-coexpressed Mn4CL and MnPKS1. Mn4CL+MnPKS2: E. coli-coexpressed Mn4CL and MnPKS2. (A) 4-Coumaric acid (1) and naringenin (2) were used as standard compounds. Selected ion chromatograms generated during liquid chromatography-electrospray ionization mass spectrometry analyses of the compounds are provided in the small panel on the right: 4-coumaric acid, m/z = 163.1; naringenin, m/z = 271.1. (B) Resveratrol (3) was used as the standard compound. A selected ion chromatogram of the compound is provided in the small panel on the right: resveratrol, m/z = 227.1. (PDF 941 kb)