Additional file 6: Figure S3. of miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1

miR-19b can also regulate TIA1 expression in CRC. (A) Schematic description of the hypothetical duplex formed by the interaction between the binding site in the TIA1 3’-UTR and miR-19b. The miR-19b seed sequence and the seed sequence binding sites in the TIA1 3’-UTR are indicated in red. All nucleotides of the seed sequence of the binding site are conserved in several species, including human, mouse, rat and rabbit. The predicted free energy values of the hybrids are indicated. (B) Quantitative RT-PCR analysis of miR-19b expression levels in the same 16 pairs of CRC and normal tissue samples. (C) Pearson’s correlation scatter plot of the fold change of miR-19b and TIA1 protein in human CRC tissue pairs. (D) Quantitative RT-PCR analysis of miR-19b levels in SW480 cells transfected with control mimic, miR-19b mimic, control inhibitor or miR-19b inhibitor. (E and F) Western blot analysis of TIA1 protein levels in SW480 cells transfected with control mimic, miR-19b mimic, control inhibitor or miR-19b inhibitor. E: representative images; F: quantitative analysis. ***P < 0.001. (TIF 917 kb)