Additional file 5: of Yerba mate (Ilex paraguariensis, A. St.-Hil.) de novo transcriptome assembly based on tissue specific genomic expression profiles

Figure S2. Experimental validation of Ilex paraguariensis transcriptome assembly. PCR primers, PCR products and sequence identity of amplified cDNA sequences of 11 predicted genes in the Ilex paraguariensis transcriptome. A: List of 11 genes identified in the transcriptome annotation, the corresponding PCR primers sequence and expected amplicon size. B: PCR products matching expected size: 100, 150 and 1200 bp. C: Amplicon DNA sequence BLASTx results showing significant sequence identity to DNA sequences from other species deposited in public databases. (PDF 690 kb)