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Additional file 5: of The molecular mechanism of LncRNA34a-mediated regulation of bone metastasis in hepatocellular carcinoma

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posted on 2019-07-27, 04:00 authored by Li Zhang, Hao Niu, Jie Ma, Bao-Ying Yuan, Yu-Han Chen, Yuan Zhuang, Gen-Wen Chen, Zhao-Chong Zeng, Zuo-Lin Xiang
The activation of IL-11 and CTGF by TGF-β in HCC cells. Figure S2. The activation of IL-11 and CTGF by TGF-β in HCC cells (n = 3). (A) SMMC-7721 and HCC-LM3 cells were treated with different amounts of TGF-β1 (approximately 0–20 ng/ml) for 2 h followed by qRT-PCR was used to detect the expression of IL-11 and CTGF; (B) IL-11 and CTGF expression was examined by qRT-PCR following treatment with 10 ng/ml TGF-β1 for the various time periods (0, 1, 2, 4, 6, 8, 12, and 24 h). Modulation of miR-34a expression in SMMC-7721 and HCC-LM3 cells. The different levels of miR-34a expression in transfected SMMC-7721 (E) and HCC-LM3 cells (F) were respectively confirmed by qRT-PCR. NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001. (TIF 606 KB)

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