40478_2020_1017_MOESM5_ESM.pdf (510.62 kB)
Additional file 5 of Ovine congenital progressive muscular dystrophy (OCPMD) is a model of TNNT1 congenital myopathy
journal contribution
posted on 2020-08-21, 04:19 authored by Joshua S. Clayton, Elyshia L. McNamara, Hayley Goullee, Stefan Conijn, Keren Muthsam, Gabrielle C. Musk, David Coote, James Kijas, Alison C. Testa, Rhonda L. Taylor, Amanda J. O’Hara, David Groth, Coen Ottenheijm, Gianina Ravenscroft, Nigel G. Laing, Kristen J. NowakAdditional file 5: Figure S4. The terminal 14 amino acids of TNNT1 are absolutely conserved across multiple mammalian species. Clustal Omega alignment of TNNT1 amino acid sequences across 6 different mammalian species. The exons that encode each region of the protein are labelled. Of note, exon 14 encodes the terminal 14 amino acids of TNNT1, which produce an intrinsically disordered domain that binds to tropomyosin [34]. Protein sequences used for alignments were as follows: sheep (Ovis aries): AMR55385 (published AA sequence from K218690 CDS), human (Homo sapiens): NP_0011196044 (NCBI RefSeq), cow (Bos taurus): NP_776899 (NCBI RefSeq), mouse (Mus musculus): NP_001264833 (NCBI RefSeq), rat (Rattus norvegicus): NP_001264191 (NCBI RefSeq), and dog (Canis lupus familiaris): XP_005616225 (NCBI predicted).
