Additional file 5 of Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription

Additional file 5: Figure S5. Deletion of IER5 enhancer1/2 using CRISPR/Cas9 in Siha and Hela cells. Genomic sequences validation of enhancer1/2 knockout by amplifying and Sanger sequencing. Sequences including the putative enhancer 1 and enhancer 2 region of IER5 gene nucleotide sequence was shown (chr1:181,074,364-181,081,980). SgRNA1 for upstream was highlighted in yellow color; sgRNA2 for downstream was highlighted in cyan color; the knockout region was highlighted in red color.