posted on 2018-03-21, 05:00authored byLesley Hoyles, Tom Snelling, Umm-Kulthum Umlai, Jeremy Nicholson, Simon Carding, Robert Glen, Simon McArthur
Figure S3. Effects of propionate upon expression and activity of typical cerebromicrovascular efflux transporter systems. (a) Surface expression of BCRP, LRP-1 and P-glycoprotein on control and propionate-treated (1 μM, 24 h) hCMEC/D3 cells (black, control, red, propionate), data are representative of three independent experiments. (b) Median fluorescence intensity of surface expression of BCRP, LRP-1 and P-glycoprotein on control and propionate-treated (1 μM, 24 h) hCMEC/D3 cells; data are mean ± SEM, n = 3 independent experiments. (c) Lack of stimulatory effect of propionate upon BCRP, data are mean ± SEM, n = 4. (d) Lack of inhibitory effect of propionate upon stimulated ATP-dependent activity of BCRP, data are mean ± SEM, n = 4. (e) Lack of stimulatory effect of propionate upon P-glycoprotein, data are mean ± SEM, n = 4. (f) Lack of inhibitory effect of propionate upon stimulated ATP-dependent activity of P-glycoprotein, data are mean ± SEM, n = 4. (PDF 437 kb)