Additional file 5: of LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer

Figure S4. Knockdown efficiency of CASC9 by Antisense LNA GapmeR or siRNA in CRC cells. (A) RT-qPCR analysis of relative CASC9 levels in HCT-116 and SW620 cells transfected with Antisense LNA GapmeRs targeting CASC9 (GapC9) or control GapmeRs (GapNC). (B) Relative CASC9 levels following biochemical fractionation of HCT-116 and SW620 cells transfected with siRNAs (left) or GapmeR (right). (C) Representative graphs of raw data for cell cycle analysis by flow cytometry (related to Fig. 2c). The data are presented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test. (TIF 1029 kb)