Additional file 5 of Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3

Additional file 5: Figure S4. Construction of transformants and effects of the truncated type ACE3-723 versus the native type ACE3-734 on the transcription of genes in the QM6a group. (A) Truncation of ACE3-734 to ACE3-723 in T. reesei QM6a, QM9414, and PC-3-7. LML 2.1 is the erasable hygromycin selection marker in T. reesei. A723 transformants carry the truncated ACE3-723 as the test strains. A734 transformants bear ACE3-734 as controls. The black square denotes the loxP site left at the C-terminus of ACE3 after the marker was excised. The primers ace3-CF and D70-4 and HG3.6 and ace3-CR were used to verify the genotype of ACE3. (B–J) Transcription of genes encoding the major cellulase (cbh1) and essential transcription factors for cellulase (ace3 and xyr1) were evaluated in T. reesei QM6a, QM9414, and PC-3-7 transformants. Three independent experiments with three biological replicates each were performed. The sar1 gene was used as the internal control for normalization. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate a significant difference (*p < 0.05, Student’s t test).