13064_2020_142_MOESM5_ESM.pdf (492.01 kB)
Additional file 5 of Cis-regulatory analysis of Onecut1 expression in fate-restricted retinal progenitor cells
journal contribution
posted on 2020-03-20, 04:55 authored by Sruti Patoori, Nathalie Jean-Charles, Ariana Gopal, Sacha Sulaiman, Sneha Gopal, Brian Wang, Benjamin Souferi, Mark M. EmersonAdditional File 5. Lineage tracing of regulatory elements reveals range in specificity. ACR2::PhiC31 and ECR42::PhiC31 were electroporated into E5 chick retinae with a PhiC31 GFP responder plasmid and CAG::Bgal and cultured for two days before harvest and staining with GFP to label cells with a history of PhiC31 expression and Bgal to label all electroporated cells. Scale bar in top right panel represents 50 μm and applies to all.
