Additional file 5: of Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding

Figure S4. GUS staining of detached leaves of Nicotiana benthamiana transgenic plants under normal and salt-stress conditions. Ninety leaf discs (diameter 0.5 cm) from fifteen 60-day-old individual plants (5 individual plants/ line, 3 lines for each construct) of PD1-PD9 and CaMV35S transgenic plants were incubated in liquid 1/2 MS medium supplemented with 200 mM NaCl at 25 °C for 3, 6, 12, 24, 48, and 72 h; leaf discs floated in liquid 1/2 MS medium were used as control. The leaf discs of PD1-PD9 and CaMV35S transgenic plants were then incubated in staining solution at 37 °C for 3 h. Finally, the samples were observed and photographed after decolorization. (JPG 3572 kb)