Additional file 5: of A functional SNP associated with atopic dermatitis controls cell type-specific methylation of the VSTM1 gene locus

Gating strategy for monocytes expressing FcγRIII, FcεRIα, or FcαR. PBMCs were stained with antibodies specific for FcγRIII (CD16), FceRIα, FcαR (CD89), and CD14 together with anti-SIRL-1, or isotype-matched control antibody. Monocytes were gated based on scatter properties (SSC-A and FSC-A) and live/dead-staining (AmCyan). Gates for FcγRIII+ monocytes, FcεRIα + monocytes, and FcαR+ monocytes are indicated in plots displaying the CD14 staining vs. the respective Fc-receptor. (PDF 297 kb)