Additional file 5: of A chicken bioreactor for efficient production of functional cytokines

Figure S5. pCSF1-Fc mono-dispersity, size and purity analysis. A) Size-exclusion chromatography multi-angled light scattering (SEC-MALS) was used to determine the molecular and mono-dispersity of pCSF1-Fc in solution. pCSF1-Fc eluted as a single peak (≥ 97% purity; As = 0.91) from a Superdex-200 Increase 10/300 GL size exclusion column with apparent molecular mass of ~ 110 ± 5 kDa and an Rs of 4.03 ± 0.4 nm (mean ± SD, n = 3). B) Elution profile of pCSF1-Fc from an RPC 5 4.6/150 ST column run. Chromatogram shows single sharp peak, with ≥98% purity. (DOCX 188 kb)