12896_2018_495_MOESM5_ESM.docx (188.57 kB)
Additional file 5: of A chicken bioreactor for efficient production of functional cytokines
journal contribution
posted on 2018-12-29, 05:00 authored by Lissa Herron, Clare Pridans, Matthew Turnbull, Nikki Smith, Simon Lillico, Adrian Sherman, Hazel Gilhooley, Martin Wear, Dominic Kurian, Grigorios Papadakos, Paul Digard, David Hume, Andrew Gill, Helen SangFigure S5. pCSF1-Fc mono-dispersity, size and purity analysis. A) Size-exclusion chromatography multi-angled light scattering (SEC-MALS) was used to determine the molecular and mono-dispersity of pCSF1-Fc in solution. pCSF1-Fc eluted as a single peak (≥ 97% purity; As = 0.91) from a Superdex-200 Increase 10/300 GL size exclusion column with apparent molecular mass of ~ 110 ± 5 kDa and an Rs of 4.03 ± 0.4 nm (mean ± SD, n = 3). B) Elution profile of pCSF1-Fc from an RPC 5 4.6/150 ST column run. Chromatogram shows single sharp peak, with ≥98% purity. (DOCX 188 kb)
