Additional file 5: Figure S5. of The anti-inflammatory Annexin A1 induces the clearance and degradation of the amyloid-β peptide

Correlation of ANXA1 expression with FPR, cytokines and Aβ. A. Scatterplot showing relationship between FPRL1/FPR2 and ANXA1 protein expression assessed by Western blotting in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 5 controls, 3 males, 2 females, range 81–97 years, mean age 86.8 ± 3 years; n = 7 AD cases, 3 males, 4 females, range 83–98 years, mean age 91.3 ± 2 years). B. Scatterplot showing relationship between FPRL1/FPR2 and ANXA1 protein expression assessed by Western blotting in the motor cortex of 5XFAD mice and wild-type littermates and (n = 6/group, males aged 12 and 26 weeks). C. Scatterplot showing relationship between FPR rs1 and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 19, males aged 12 and 26 weeks). D. Scatterplot showing relationship between ANXA1 protein expression assessed by Western blotting and TNFα expression measured by ELISA in in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 8 controls, 5 males, 3 females, range 40–82 years, mean age 67.1 ± 6 years; n = 10 AD cases, 7 males, 3 females, range 42–98 years, mean age 72.5 ± 6 years). E. Scatterplot showing relationship between Tnf and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 18, males aged 12 and 26 weeks). ***p < 0.001, r 2 = 0.5779. F. Scatterplot showing relationship between Il6 and ANXA1 mRNA expression assessed in the frontal cortex of 5XFAD mice and wild-type littermates by qPCR (n = 19, males aged 12 weeks and 26 weeks). ****p < 0.0001, r 2 = 0.6352. G. Scatterplot showing relationship between ANXA1 protein expression assessed by Western blotting and Aβ1–40 expression measured by ELISA in the frontal cortex of neurologically healthy controls and sporadic Alzheimer’s patients (n = 10 controls, 6 males, 4 females, range 40–97 years, mean age 71.5 ± 6 years; n = 12 AD cases, 8 males, 4 females, range 42–98 years, mean age 74.4 ± 5 years). H. Scatterplot showing relationship between Aβ and ANXA1 protein expression assessed by Western blotting in the cortex of 5XFAD mice (n = 11, males aged 12 and 26 weeks). (PDF 111 kb)