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Additional file 5: Figure S5. of Blockade of autophagy reduces pancreatic cancer stem cell activity and potentiates the tumoricidal effect of gemcitabine

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posted on 2015-10-12, 05:00 authored by Ming-Chen Yang, Hao-Chen Wang, Ya-Chin Hou, Hui-Ling Tung, Tai-Jan Chiu, Yan-Shen Shan
OPN triggered STAT3, ERK, and NF-κB activation, but not AKT, JNK, and p38 MAPK in pancreatic cancer cells. (A) PANC-1 and MIA PaCa-2 cells were treated with OPN (100 ng/mL) for the indicated time points. The cell lysates were prepared and subjected to Western blotting using the indicated antibodies. (B) PANC-1 and MIA PaCa-2 cells were incubated with OPN (100 ng/mL) for the indicated time points. Cells were lysed and fractionated, and the levels of NF-κB p65 in both cytosolic and nuclear fractions were detected by Western blotting. β-Actin serves as a cytosolic marker, and LAMIN A/C serves as a nuclear marker. (For detail, please see Additional file 8). (TIFF 2417 kb)

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