Additional file 5: Figure S5. of A biomaterials approach to influence stem cell fate in injectable cell-based therapies

Showing cellular ALP activity levels of hMSCs at different time points following ejection at 10 μl/min via 30G needles, and cultured in bipotential media. (A) Cellular ALP analysed 2, 4 and 7 days post induction. Values are mean ± SD (n = 3 in two donors). Statistically significant differences in ALP levels relative to control (Friedman test with Dunn’s post-hoc test: *p < 0.05. (B) Cellular ALP values normalised to DNA content (mean ± SD, n = 3 in two donors). (C) Normalised cellular ALP levels in ejected versus directly plated hMSCs suspended within collagen and ECM. ‘Ejected’ cells ejected at 10 μl/min, and ‘plated’ cells were 60% of the initial cell number directly plated (mean ± SD, n  =  3 in two donors). (D) DNA content of hMSCs in ejected versus directly plated samples suspended within collagen and ECM (mean ± SD). (E) Representative immunofluorescent staining of human osteocalcin (OCN) and nuclei counterstained with DAPI (blue) to confirm osteogenic differentiation of hMSCs. Directly plated and ejected hMSCs (via 30G needles at 10 μl/min) cultured in bipotential media at 21 days post induction (scale bar = 50 μm). (PDF 926 kb)