Additional file 5: Figure S4. of Vitamin D3/VDR resists diet-induced obesity by modulating UCP3 expression in muscles

The presence of VDR transcripts was confirmed by RT-PCR analysis of C2C12 cells, L6 cells, H-EMC-SS cells and the rectus femoris muscle. PCR products of the expected sizes were detected by agarose gel electrophoresis, suggesting that the transcripts were present in each cell line. Beta actin was used as a positive control for PCRs. (PPTX 49 kb)