Additional file 5: Figure S4. of Remodeling of lipid bodies by docosahexaenoic acid in activated microglial cells

Mitochondrial membrane potential of microglial cells exposed to DHA. A. Change in mitochondrial membrane potential in N9 microglial cells exposed to DHA and LPS for 6 h. Mitochondrial membrane potential of N9 microglial cells treated with DHA (50 μM) for 6 h. Following treatment, the cells were incubated with TMRE (50 nM) for 20 min, after which the media was refreshed and cells were imaged under a fluorescence microscope. Three fields were imaged per treatment, and ten cells were imaged per field. Shown are average fold increase in intracellular relative fluorescence intensities ± SEM as compared to untreated control (set to 1) from two independent experiments. *p < 0.01; **p < 0.001 B. Change in mitochondrial membrane potential in N9 microglial cells exposed to DHA (5–50 μM) for 24 h. Following treatment, cells were incubated with TMRE (50 nM) for 20 min, after which the media was refreshed and cells were imaged under a fluorescence microscope. Four fields were imaged per treatment, and two cells were imaged per field. Shown are average fold increase in intracellular relative fluorescence intensities ± SEM as compared to untreated control (set to 1) from three independent experiments. Cells imaged in the absence of TMRE and cells treated with FCCP were negative controls. *p < 0.01. (TIF 1214 kb)