Additional file 5: Figure S4. of Neonatal monocytes exhibit a unique histone modification landscape

Differential binding of H3K4me3 at pro-inflammatory cytokine promoters during development results in differences in gene expression. (A) Increased binding of H3K4me3 at the promoter site of IL1B in adult monocytes is associated with increased IL1B mRNA and protein expression after LPS stimulation. (B) Increased binding of H3K4me3 at the promoter site of TNF in adult monocytes is associated with increased TNF mRNA and protein expression after LPS stimulation. A = adult, T = term. H3K4me3 peaks display the read coverage for H3K4me3 from one of the representative replicates from each group. Black bars display the H3K4me3 consensus peaks after only peaks present in at least two replicates are combined and analyzed. For the mRNA studies, adult n = 5, term n = 5. For the protein studies, adult n = 4, term n = 4. *p < 0.05, **p < 0.01. (PDF 215 kb)