Additional file 5: Figure S4. of A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling

Testing opsin coupling to endogenous Gs. To test potential opsin coupling to endogenous Gs, HEK293T cells were transfected with Glo22F and opsins treated with pertussis toxin, and exposed to 470 nm light for n = 3 replicates. The signal for each trial was normalized to pre-flash. The maximum cAMP post-flash was recorded for each trial. Graphs show mean cAMP response +/- SEM at varying irradiance for each opsin. RL3Am and RL3m6L2 exhibited responses that satisfied our statistical criteria (fitted response curves are shown). Error bars smaller than symbols are not shown. (PDF 454 kb)