Additional file 5: Figure S3. of Pangenome analysis of Bifidobacterium longum and site-directed mutagenesis through by-pass of restriction-modification systems

Restriction analysis of B. longum genomic DNA. Restriction analysis of genomic DNA from B. longum subsp. longum NCIMB 8809 and B. longum subsp. longum CCUG 30698. Lane 1, molecular weight marker (Bioline). Lane 2: Unrestricted total DNA from B. longum subsp. longum NCIMB 8809, Lane 3 and 4 total B. longum subsp. longum NCIMB 8809 DNA restricted with lane 3 EcoRII and lane 4 PstI. Lane 5: Unrestricted total DNA from B. longum subsp. longum CCUG 30698, Lane 6 and 7 total B. longum subsp. longum CCUG 30698 DNA restricted with lane 6 EcoRII and lane 7 PstI. (PDF 93 kb)