Additional file 4: of circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma

Figure S3. circPTN rescues the inhibition of self- renewal by miR-145-5p in U87-GSC and U251-GSC. A. Results of western blot assay showed that circPTN could rescue the inhibition of SOX9 expression by miR-145-5p in U87-GSC and U251-GSC. B. Upper: Images of tumor sphere formation assay (200 cells/well) at day 14; scale bar, 50 μm; Lower: Analyses of tumor sphere formation results indicated that circPTN could rescue the inhibition of self-renewal of U87-GSC and U251-GSC by miR-145-5p, n = 10, *p < 0.05, t test. C. Results of western blot assay showed that circPTN-145-mut failed to rescue the inhibition of SOX9 expression by miR-145-5p in U87-GSC and U251-GSC. D. Upper: Images of tumor sphere formation assay (200 cells/well) at day 14; scale bar, 50 μm; Lower: Analyses of tumor sphere formation results indicated that circPTN-145-mut failed to rescue the inhibition of self-renewal of U87-GSC and U251-GSC by miR-145-5p, n = 10, *p < 0.05, t test. (TIF 2388 kb)