Additional file 4 of Towards single-cell ionomics: a novel micro-scaled method for multi-element analysis of nanogram-sized biological samples

Additional file 4. Manganese distribution in a barley cross section. Manganese distribution in a barley cross section, 2 cm behind the root tip, analysed by Laser Ablation-ICP-MS as 55Mn. The signal intensity is displayed as a heat map (upper image) where red represent the strongest intensities and purple the weakest. The signals in the graph below, stem from five transversal lines extracted from the heat map data (dotted line), showing average signals ± SE from the left side of cortex (minus epidermis), stele and right side of cortex (minus epidermis) (st = stele, co = cortex, ep = epidermis).