Additional file 4: of The p52 isoform of SHC1 is a key driver of breast cancer initiation

Figure S4. Western blot analysis of ERK1/2 stimulation. Primary mammary epithelial cells isolated from wild-type or p52ShcKO rats were serum-starved overnight and stimulated for 5 min with b-estradiol at the indicated concentrations. Western blot analysis is carried out with phosphospecific ERK antibodies. Positions of phospho-ERK 1 and 2 are indicated by arrows. Shown is a representative experiment out of 3. (TIF 105 kb)