Additional file 4: of TRIB2 functions as novel oncogene in colorectal cancer by blocking cellular senescence through AP4/p21 signaling

Figure S4. AP4 promotes tumorigenesis in colorectal cancer. a mRNA expression of AP4 in 15 pairs of human primary CRC tissues (normal and Tumor). b Western blot analysis of AP4 and p21 in SW48 and LoVo cells transfected with AP4 specific siRNA (#1 and #2) or siNC. c RT-PCR analysis of AP4 and p21 in SW48 and LoVo cells transfected with AP4 specific siRNA (#1 and #2) or siNC. d CCK8 analysis of cell proliferation capacity in AP4-knockdown or control SW48 and LoVo cells. e SA-β-gal staining analysis of SW48 and LoVo cells transfected with AP4 specific siRNA (#1 and #2) or siNC (dox, 0.25 μmol/l, 48 h). left panel, representative images of SA-β-gal staining; right panel, percentage of SA-β-gal-positive cells. Results are presented as mean ± SD from three independent assays, * p < 0.05, ** p < 0.01, *** p < 0.001, t-test. (TIF 2707 kb)