Additional file 4: of Suitability of 3D human brain spheroid models to distinguish toxic effects of gold and poly-lactic acid nanoparticles to assess biocompatibility for brain drug delivery

Figure S2. Effect of NP on expression of genes related to ROS regulation in 3D LUHMES. Graphs showing the relative expression of SOD1, NF2L2, NFR1, GSTO1 and SOD2 after exposure to Au-SC (6 μg/mL), Au-PEG (20 μg/mL) and PLA-NP (20 μg/mL) for 72 h normalized to the expression of the genes in the untreated control spheroids. Data was collected from two independent experiments with total eight biological replicates and represents fold changes (FC ± SEM). Only four replicates were used for Au-SC treated samples. One-way ANOVA with Dunnett’s multiple comparisons post-test was used to analyze the statistical significance. (TIF 86 kb)