Additional file 4: of Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength

Validation of mitotic arrest following PRPF8 depletion with two independent siRNAs. a U2OS osteosarcoma cells depleted of PRPF8 using two independent siRNAs and monitored by phase-contrast time-lapse microscopy spend significantly longer in mitosis (measured from nuclear envelope breakdown (NEB) to chromatin decondensation), as opposed to control cells, in which mitosis lasts, on average, <60 min. Statistically significant pairwise comparisons are shown (***p < 0.001). b Introduction of properly spliced and processed mRNA into Control siRNA-treated cells has no effect on mitotic index. Control siRNA-treated cells were transfected with properly spliced and processed mRNA purified from whole cells and analyzed for mitotic index. Plots represent the mean from two independent experiments Âą standard error of the mean. n.s. not significant. (PDF 77 kb)