13395_2020_226_MOESM4_ESM.pdf (4.49 MB)
Additional file 4 of High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers
journal contribution
posted on 2020-03-24, 04:56 authored by Sebastian Kallabis, Lena Abraham, Stefan Müller, Verena Dzialas, Clara Türk, Janica Lea Wiederstein, Theresa Bock, Hendrik Nolte, Leonardo Nogara, Bert Blaauw, Thomas Braun, Marcus KrügerAdditional file 4: : Suppl. Figure 4: Increased fluorescence of tropomyosin-1 stained EDL type IIb compared to TA type IIb myofibers. A) Co-immunostaining of tropomyosin-1 (TPM1) and myosin-4 in muscle cryosections showing the TA (left to the dashed line) and EDL (right to the dashed line) muscle. Type IIb positive myofibers are marked with “a” (TA) or “b” (EDL). B) Quantitative analysis of the TPM1 fluorescence signal in type IIb positive fibers of the TA or the EDL muscle. Significance was tested by two-sided t-testing (n = 40). C) Co-immunostaining of titin (TTN) and myosin-4 in muscle cryosections showing the TA (left to the dashed line) and EDL (right to the dashed line) muscle. Type IIb positive myofibers are marked with “a” (TA) or “b” (EDL). B) Quantitative analysis of the TTN fluorescence signal in type IIb positive fibers of the TA or the EDL muscle. Significance was tested by two-sided t-testing (n = 30)