Additional file 4: of Functional characterization of the cytochrome P450 monooxygenase CYP71AU87 indicates a role in marrubiin biosynthesis in the medicinal plant Marrubium vulgare
journal contributionposted on 25.03.2019 by Prema Karunanithi, Puja Dhanota, J. Addison, Shen Tong, Oliver Fiehn, Philipp Zerbe
Any type of content formally published in an academic journal, usually following a peer-review process.
Figure S2. GC-MS analysis of reaction products derived from Nicotiana benthamiana co-expression assays of MvCPS1 and MvELS the P450 candidates Mv1270, Mv1545, Mv4213, Mv6504, and Mv3392, respectively. Shown are extracted ion chromatograms (m/z 151) of the diterpenoid products 9,13-epoxy labd-14-ene 1, labda-13(16),14-dien-9-ol 2, unidentified diterpene 3, 9,13-epoxy labd-14-ene-18-ol 4, and 9,13-epoxy labd-14-ene-19-ol 5. The suppressor of RNA silencing p19 was included in all co-expression assays. (A) Co-expression of MvCPS1, MvELS and MvCYP71AU87, (B) Co-expression of MvCPS1 and MvELS, (C-F) Co-expression of MvCPS1 and MvELS with the P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively), (G-J) Co-expression of MvCPS1 and MvELS with MvCYP71AU87 and the individual P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively. (K) Expression of MvCYP71AU87 only. (PDF 264 kb)