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Additional file 4: of Functional characterization of the cytochrome P450 monooxygenase CYP71AU87 indicates a role in marrubiin biosynthesis in the medicinal plant Marrubium vulgare

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posted on 25.03.2019 by Prema Karunanithi, Puja Dhanota, J. Addison, Shen Tong, Oliver Fiehn, Philipp Zerbe
Figure S2. GC-MS analysis of reaction products derived from Nicotiana benthamiana co-expression assays of MvCPS1 and MvELS the P450 candidates Mv1270, Mv1545, Mv4213, Mv6504, and Mv3392, respectively. Shown are extracted ion chromatograms (m/z 151) of the diterpenoid products 9,13-epoxy labd-14-ene 1, labda-13(16),14-dien-9-ol 2, unidentified diterpene 3, 9,13-epoxy labd-14-ene-18-ol 4, and 9,13-epoxy labd-14-ene-19-ol 5. The suppressor of RNA silencing p19 was included in all co-expression assays. (A) Co-expression of MvCPS1, MvELS and MvCYP71AU87, (B) Co-expression of MvCPS1 and MvELS, (C-F) Co-expression of MvCPS1 and MvELS with the P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively), (G-J) Co-expression of MvCPS1 and MvELS with MvCYP71AU87 and the individual P450 Mv1545, Mv4213, Mv6504, and Mv3392, respectively. (K) Expression of MvCYP71AU87 only. (PDF 264 kb)

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