Springer Nature
Browse
12896_2018_453_MOESM4_ESM.pdf (48.88 kB)

Additional file 4: of Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes

Download (48.88 kB)
journal contribution
posted on 2018-06-27, 05:00 authored by Martinus Daas, Bart Nijsse, Antonius van de Weijer, Bart Groenendaal, Fons Janssen, John van der Oost, Richard van Kranenburg
Figure S2. Congo red assays of cellulase expressing G. thermodenitrificans cultures. Congo red assays of G. thermodenitrificans cultures grown on LB2 medium with 1% carboxymethylcellulose. Each culture produces a different cellulase. Ø: empty plasmid (pNW33n) control; CelA: GH5 endoglucanase CelA (Geobacillus 70PC53); CelK: GH9 exoglucanase (C. thermocellum); GE40wt: GH5 endoglucanase (Geobacillus metagenome derived) containing its native signal peptide; GE40: GH5 endoglucanase (Geobacillus metagenome derived). (PDF 48 kb)

Funding

BE-Basic foundation

History

Usage metrics

    BMC Biotechnology

    Categories

    Keywords

    GeobacillusMetagenomeβ-XylosidaseCellulaseCBP

    Licence

    CC BY + CC0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC