Additional file 4: of Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells

Figure S3. Gene expression of (A) free fatty acid receptor 1 (FFAR1) and (B) free fatty acid receptor 4 (FFAR4) in bovine ovarian cells. Total mRNA was extracted from the ovarian cortex (CX), thecal cells (TH), granulosa cells (GC) and cumulus cells (CC). Total mRNA was then reverse-transcribed and real-time RT-PCR was performed. The geometric mean of two housekeeping genes (RPL19- ribosomal protein L19 and RPS9- ribosomal protein S9) was used to normalize gene expression. Results of 2 to 4 independent samples are presented as means ± SEM. Bars with different superscripts are significantly different (p < 0.05). (TIF 59 kb)