40425_2018_371_MOESM4_ESM.pdf (2.51 MB)
Additional file 4: of Dexamethasone-induced immunosuppression: mechanisms and implications for immunotherapy
journal contribution
posted on 2018-06-11, 05:00 authored by Amber Giles, Marsha-Kay Hutchinson, Heather Sonnemann, Jinkyu Jung, Peter Fecci, Nivedita Ratnam, Wei Zhang, Hua Song, Rolanda Bailey, Dionne Davis, Caitlin Reid, Deric Park, Mark GilbertFigure S4. Increased co-stimulation ameliorates the inhibitory effects of dexamethasone. Negatively-selected healthy donor T cells were cultured with 5 μg/mL αCD3 and increasing concentrations of CD80 in the presence of vehicle or dexamethasone. A-B. CD8 T cells cultured with vehicle (A) or dexamethasone (B). Flow cytometry plots showing proliferation of cells cultured with the indicated concentration of CD80 (left) and total numbers of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TTE) T cells following four days of culture (right) are shown. Differentiation subsets were assessed by CD45RO and CCR7 staining. Each condition was plated in duplicate, and data are representative of three independent experiments. Data were analyzed with an unpaired, two-tailed T Test. (PDF 2573 kb)