Additional file 4: of Combination treatment strategy for pancreatic cancer involving the novel HDAC inhibitor MPT0E028 with a MEK inhibitor beyond K-Ras status

Figure S4. Overexpressed HDAC4, HDAC6, or MEK can reverse the apoptotic effect of combination in PANC-1 cells. PANC-1 cells were transfected with empty vector, HDAC1, HDAC4, HDAC6, or GFP-MEK plasmid overnight and then co-treated with 2 μM MPT0E028 and 20 μM PD98059 for 48 h. (A) The protein expression of HDAC1, HDAC4, HDAC6, GFP, and (B) PARP. Actin was used as an internal control. E, MPT0E028; PD, PD98059. (TIF 1669 kb)