Additional file 4: of Ancient role of vasopressin/oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm

Characterisation of antisera to asterotocin and the asterotocin receptor using an enzyme-linked immunosorbent assay (ELISA). (A) Incubation of rabbit antiserum (blue) at dilutions between 1:500 and 1:16,000 with 0.1 nmol of asterotocin antigen peptide per well reveals that the antigen is detected at dilutions between 1:500 and 1:4000 by comparison with absorbance measurements for pre-immune rabbit serum (undiluted; red) with 0.1 nmol antigen peptide (Lys-asterotocin) per well. All data points are mean values from three replicates. (B) Incubation of guinea pig antiserum (green) at dilutions between 1:500 and 1:16,000 with 0.1 nmol of asterotocin receptor antigen peptide per well reveals that the antigen is detected across the full range of dilutions tested by comparison with absorbance measurements for pre-immune guinea pig serum (undiluted; purple) with 0.1 nmol antigen peptide per well. All data points are mean values from three replicates. (TIF 5347 kb)