Additional file 4 of An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Additional file 4: Fig. S4. Verification of Bdo_02540 GD B. dothidea HTLW03 transformants. The Bdo_02540 GD transformant (ΔBdo_02540–1) was analyzed with four PCR amplifications and a Southern blot. aΔBdo_02540–1 had the correct upstream and downstream fragments, lacked the ORF fragment, and had a longer whole-length fragment than the WT control. b Southern blot analysis of the hph insertion loci.