Additional file 4: of An APETALA1 ortholog affects plant architecture and seed yield component in oilseed rape (Brassica napus L.)

Figure S1. Relative expression of Bna.AP1 in BC2F3 lines homozygous for Bna.TFL1.A10 missense mutation (Guo et al., 2014). (A) Combined expression of Bna.AP1 (B) Paralog-specific (Bna.AP1.A02). aa-M4: Genotype carrying Bna.TFL1.A10 missense mutation (M4 generation); aa-BC2F3: Genotype having homozygous Bna.TFL1.A10 missense mutation in a BC2F3 generation; AA: Genotype carrying Bna.TFL1.A10 wildtype allele in BC2F3 generation; Express617: Control. Expression levels of target genes were normalized against Bna.Actin total expression. For all genotypes tissue (SAM) sampling was done between zeitgeber 8 h and 9 h. Three biological replicates and three technical replicates were used for each genotype. Error bars: standard error of the mean for biological replicates. The mean comparison between the genotypes for the investigated traits was performed by ANOVA test (P value = 0.0001), while the grouping was done using the LSD test (α ≤ 0.05) in R package ‘Agricolae’ version 1.2–8. (PPTX 522 kb)